Preparation of rough microsomes from rat liver.
نویسنده
چکیده
This protocol describes how to prepare rat liver rough microsomes that contain undegraded membrane-bound polysomes and can function very well in an in vitro translation system. It uses endogenous ribonuclease inhibitor in all steps, avoiding pelleting rough microsomes in all steps and sacrificing good recovery.
منابع مشابه
An Improved Cell Fractionation Procedure for the Preparation of Rat Liver Membrane-bound Ribosomes
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عنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2014 8 شماره
صفحات -
تاریخ انتشار 2014